QuestOmics
Human Gut Microbiome Platform

About

I built QuestOmics around the questions, not the pipelines.

QuestOmics is a human gut microbiome analysis platform. I focused it on the questions researchers actually ask, not the pipelines that answer them, so you can spend your time on the biology instead of the plumbing.

Sandro Valenzuela

BSc in Bioinformatics · PhD in Infant Gut Microbiome, University of Helsinki

Why I built it

Less plumbing, more biology

Microbiome research moves slowly when most of the work is plumbing. Stitching together tools, formats, and clusters eats the time that should go into asking biological questions. QuestOmics removes that friction: you describe what you want to learn, and the platform runs the right, reproducible analyses for you.

Question-driven analysis

You pick the question

Instead of picking a pipeline and figuring out what it can tell you, you pick a question. “What organisms dominate this community?”, “Are AMR genes present?”, “How does this cohort compare to published studies?” QuestOmics then activates the curated, compatible analyses for it. Incompatible options stay disabled. No manual tool selection. No glue code.

What you can analyze

Built for human gut microbiome data

Supported inputs: shotgun metagenomes (short and long reads), 16S rRNA amplicons, bacterial isolates, and pre-assembled contigs or MAGs.

Taxonomic profiling

16S and shotgun metagenomic composition.

AMR / resistome screening

Detection of resistance genes across the community.

Functional pathway profiling

Which metabolic pathways are active across your samples.

Differential abundance

Which species differ between groups, adjusting for covariates.

Cohort comparison

Your samples next to thousands of published gut metagenomes.

Diversity

Within and between samples; the standard ecology metrics.

How I keep it honest

Cited interpretation and reproducible runs

Grounded in literature

Tables are not interpretation. Every analysis is paired with Gut Assistant, an AI layer built specifically for microbiome science. It reads from a curated library of peer-reviewed papers and cites only what it can point you to.

Reduced Faecalibacterium prausnitzii in your samples is consistent with the depleted butyrate-producer signature reported in inflammatory states [1].
[1]Sokol et al., PNAS 2008

Reproducible by design

Every run is sealed and version-pinned, so anyone can reproduce it. Software versions are recorded at execution time, and methods text is auto-generated per run, ready to paste straight into a Materials & Methods section.

Software versions
MetaPhlAn4.0.6
Bowtie22.5.1
marker DBmpa_vJun25

Your data stays yours

What happens to your data

Your sequencing data and analysis results belong to you and your study. They are never used for external research, never pooled into external datasets, never sold, and never used to train external models. Data is stored on private, secure hardware in the EU.

Who’s behind it

Built and run by one person

Sandro Valenzuela
University of Helsinki

Sandro Valenzuela

BSc in Bioinformatics · PhD in Infant Gut Microbiome, University of Helsinki

QuestOmics is a one-person labor of love right now, built by a microbiome scientist who got tired of the plumbing getting in the way of the questions. If that resonates, I’d love for you to take it for a spin: open a workspace, upload a dataset, and ask your first question. And once you’re inside, reach out through Gut Assistant. I read every message and I’d genuinely like to hear what you’re working on.

Prefer email? I’d love to hear from you, so write to me anytime at svalenzuela@questomics.app.

Let’s talk

Have a question, a dataset, or just feedback?

Tell me what you’re working on, whether it’s a question, a dataset, or something you’re stuck on. I read every message and reply personally.

or copy svalenzuela@questomics.app

Ready when you are

Get started

Create a workspace, upload a dataset, and ask your first biological question.